Biotransformation of monoterpenes using Streptomyces strains from the rhizosphere of Inga edulis Martius from in an Amazonian urban forest fragment.

To investigate the biocatalytic potential of Amazonian actinomycetes for monoterpenes biotransformation. To carry out the present study, eleven actinomycetes of the genus Streptomyces isolated from inga-cipó (Inga edulis Mart.) rhizospheres were tested for their ability to bioconvert the substrates R-(+)-limonene, S-(-)-limonene, 1S-(-)-α-pinene, and (-)-ß-pinene as sole carbon and energy source. According to gas chromatography-mass spectrometry analysis, three strains, LabMicra B270, LaBMicrA B310, and LaBMicrA B314, were able to biotransform 1S-(-)-α-pinene after 96 h of growth. However, Streptomyces LaBMicrA B270 was the most promising since it converted after only 72 h all the 1S-(-)-α-pinene mainly into cis-verbenol (74.9±1.24%) and verbenone (18.2±1.20%), compounds that have important biological activities and great industrial interest as additives in foods and cosmetics. These findings can stimulate the development of natural aromas using naturally abundant monoterpenes, ratify the potential of microorganisms from almost unexplored niches such as the Amazonian rhizosphere, and reinforce the importance of preserving those niches.

Interaction between residual feed intake and thermal environment on performance, nitrogen balance, ingestive behavior and carcass yield of dorper lambs.

Residual feed intake (RFI) is a nutritional variable used in genetic improvement programs, the relationship between the environment and the availability of energy and protein in the diet has not yet been explored. Thus, the aim was to evaluate interactions between RFI and thermal environment on performance, nitrogen balance, ingestive behavior and carcass yield of Dorper lambs receiving diets containing different concentrate levels. Dorper lambs (male, n = 64, 17.83 ± 2.43 kg and 110 ± 10 days of age) were confined individually for 40 days for RFI classification. Lambs were separated into positive RFI (n = 30) and negative RFI (n = 30) and remained confined for another 60 days. The animals were distributed in a randomized block design, with a 2 × 2 × 3 factorial scheme, with 2 confinement environments (full sun or shade), 2 groups of feed efficiency (RFI positive or RFI negative) and three diets containing different concentrate levels (30, 45 and 60%), with 5 replications in each treatment. Isolated effects of concentrate level were observed for dry matter intake and digestibility, feeding, rumination, idle and chewing times, feeding efficiency, ingested, excreted and absorbed nitrogen, and on cooling losses, hot and cold carcass yield (P < 0.05). There was an effect of environment × concentrate interaction on performance, retained nitrogen and nitrogen balance (P < 0.05). There was an effect of RFI × environment interaction on the dry matter rumination efficiency, hot and cold carcass weight (P < 0.05). Under experimental conditions, RFI did not influence the productive performance of Dorper lambs. Interactions between environment and diet indicate better performance for Dorper lamb confined in the shade and receiving a higher proportion of concentrate. Animals with negative RFI show better performance and carcass weight when confined in shade, while animals with positive RFI showed better responses to these variables when confined in full sun.

Effect of different levels of sodium in water on performance, carcass yield, and meat quality of slow-growing chickens.

The aim of this study was to evaluate the effects of sodium (Na) levels in the drinking water on the performance, carcass yield, and meat quality of slow-growing chickens. A completely randomized design with 4 treatments (4.90; 32.30; 60.53; and 101.0 mg/L of Na in water) and 6 replications, with 20 birds per experimental unit, was adopted. The treatments are evaluated in the following: 10 to 25, 10 to 39, and 10 to 54 days. For slow-growing chickens from 10 to 25 days, the Na levels in the drinking water promoted a quadratic effect for water and feed intake (p<0.05). The inclusion of Na in the drinking water offered to slow-growing chickens from 10 to 39 days resulted in a reduction in voluntary water intake (p<0.05). For slow-growing chickens from 10 to 54 days, the Na levels in the drinking water promoted a quadratic effect for water intake and feed conversion (p<0.05). After 54 days, the slow-growing chickens were slaughtered and it was observed that the Na inclusion in the drinking water offered to slow-growing chickens provided a quadratic effect for cold carcass, breast, and kidney weights and for kidney and liver yields (p<0.05). Liver weight was reduced with increasing Na levels in the drinking water (p<0.05). For the breast cut, the Na levels in the drinking water promoted a quadratic effect for pH24h, drip loss, cooking loss, protein, and fat and an increase in shear force (p<0.05). For the thigh cut, the Na levels in the drinking water increased the pH24h and reduced drip loss and shear force (p<0.05), and a quadratic effect was observed for moisture and fat (p<0.05). Levels of up to 60.53 mg/L of Na promote an increase in feed intake, which provided a higher breast weight and protein content with reduced fat and drip loss.

Grape skin flour obtained from wine processing as an antioxidant in beef burgers.

The objective of this study was to determine the best level of wine making by-product meal (WBM) as a natural antioxidant to replace butylhydroxytoluene (BHT) in beef burger stored at -20 °C for up to 120 days. The treatments consisted of control (basic formulation - BF, without antioxidant); BF with BHT; and BF with WBM0.5, WBM1.0, WBM1.5, and WBM2.0, with 0.5, 1.0, 1.5 and 2.0 g WBM/100 g BF, respectively. Up to 60 days of storage, the lipid oxidation value between BHT and WBM0.5 treatments did not differ and were lower than the values presented by the other treatments. On day 90 and 120, the lipid oxidation values of treatments BHT, WBM0.5, and WBM1.0 did not differ and were lower than the values presented by WBM1.5 and WBM2.0 treatments. Burgers from all treatments with WBM inclusion had crude fiber values above 3 g/100 g. WBM1.5 and WBM2.0 treatments had the worst scores for appearance, aroma, juiciness and tenderness, in addition to the highest cooking losses. WBM can be used at up to 1 g/100 g to replace BHT in frozen beef burgers. Higher levels of WBM inclusion increased lipid oxidation and negatively affected the sensory quality of burgers.

Tannin as a modulator of rumen microbial profile, apparent digestibility and ingestive behavior of lactating goats: A preliminary metagenomic view of goats adaptability to tannin.

The aim with this study was to use interdisciplinary techniques and visions in order to identify the modulating effect of tannins on adaptive factors in lactating goats with different genetic patterns, through the assessment of digestibility and nutrient intake, ingestive behavior and rumen metagenome. We used in this study 8 of the Repartida ecotype and 6 of the Canindé breed goats, on average five years old, distributed in a completely randomized design, in a 2 × 2 factorial design, with two genetic groups and two diets. The applied diets were: basal diet and inclusion of 5% commercial tannin extract in basal diet. Intake and apparent digestibility of nutrients were evaluated, based on the quantification of the offered feed and refusals, and bromatological evaluation of samples of the offered feed, refusals and feces. Behavioral data were collected in 24-h continuous visual observations. The ruminal fluid was collected and DNA extraction, sequencing, and evaluation of relative abundance of the rumen microbiome were performed. The data obtained were analyzed statistically, through analysis of variance with 5% significance and, when necessary, a comparison of means test was applied. In this preliminary findings was observed that the genetic group factor caused changes in the number of chews and the relative abundance of microorganisms (P = 0.0290 and P = 0.0051). The diet factor influenced digestibility, which better values were observed for the tannin diet (P = 0.0049), in addition, it promoted changes in the rumen microbiota, with a beneficial modulatory characteristic. The inclusion of 5% tannin extract from Acacia mearnsii modulates the rumen microbiome, improving the apparent digestibility of nutrients without affecting the feed intake of goats from the Repartida and Canindé genetic groups.

Sodium hydroxide or urea pretreatment of acerola (Malpighia emarginata) fruit residue increases dry matter degradability and reduces methane production in in vitro rumen fermentation.

The aim of this study was to evaluate the effect of adding different concentrations of either urea or NaOH in dehydrated acerola (Malpighia emarginata) fruit residue (DAFR) on chemical composition, in vitro rumen degradability, and gas and methane production. A completely randomized design was used with the following seven treatments: control, without chemical treatment, or pretreatment of DAFR with urea or NaOH at 20, 40, or 60 g/kg dry matter (DM). DM degradability and gas and methane production of DAFR were evaluated by semi-automated in vitro gas production technique. DAFR treated with urea or NaOH at concentrations of 40 and 60 g/kg DM decreased its neutral detergent fiber (P = 0.0115) and lignin (P < 0.0001) content, and this reduction was greater with the highest concentration (60 g/kg DM). In all tested concentrations, urea and NAOH were effective to increase the DM effective degradability of DAFR compared with the control treatment, although treatments with a concentration of 60 g/kg DM presented the highest values (P < 0.0001). Treatment of DAFR with NaOH or urea at 60 g/kg DM promotes greater lignin solubilization and DM degradability and lower gas and methane production in in vitro rumen fermentation.

Diets containing cunhã (Clitoria ternatea L.) hay and forage cactus (Opuntia sp.) meal on production and meat quality of Boer crossbred goat.

The aim of this study was to evaluate the effect of association of forage cactus meal (CM) and cunhã hay (CH) on carcass traits, meat quality, and fatty acid (FA) profile of goats. Twenty-four goats were finished on diets composed with the association of CM and CH (83% CH + 17% CM, 67% CH + 33% CM, or 50% CH + 50% CM), corresponding to 60% of the total diet; the remainder was composed of 10% elephant grass and 30% concentrate. The control treatment consisted of 70% elephant grass and 30% concentrate. Carcass yield and commercial cut weight were higher in the animals fed CH and CM at any concentration (P < 0.05). Animals fed with intermediate and high CM content resulted in meat with better proportions of unsaturated FA (P = 0.0281), desirable FA (P = 0.0024), omega 6/omega 3 ratio (P = 0.0340), and hypo-/hypercholesterolemic index (P = 0.0140). The inclusion of CH and CM provided carcass yield and FA profile more favorable for consumer market and human health.

Use of bean meal (Phaseolus vulgaris L.) in goat rations for meat production.

Bean meal (Phaseolus vulgaris L.) may be a viable alternative in ruminant feeding, mainly as a source of protein due to its high crude protein content. The aim of this study was to evaluate the effect of substitution of cottonseed cake (Gossypium hirsutum) with different levels of bean meal in goat feedlot rations on dry matter intake, digestibility, feeding behavior, performance, carcass characteristics, and hematological parameters. Twenty castrated male Repartida goats with an average initial weight of 14.7 ± 2.0 kg were allocated in a randomized block design with four treatments. The initial body weight was used to define the blocks. The treatments were different substitution levels of cottonseed cake by bean meal (0, 27.75, 64.63, and 100%) in the concentrate on a dry matter basis. The diets had a roughage:concentrate ratio of 50:50. Chopped elephant grass (Pennisetum purpureum) was used as roughage, while the concentrate was composed of ground corn, mineral mix, and different levels of bean meal and cottonseed cake. There were no treatment effects on dry matter intake, feed conversion, slaughter weight, carcass weight and yield, non-carcass component yield, and hematological parameters (P > 0.05). However, for both dry matter digestibility (P = 0.001) and daily weight gain (P < 0.001), there was a negative quadratic relationship between cottonseed cake and bean meal content in the diet. Bean meal can be used in goat diets to replace cottonseed cake as the only source of protein concentrate, since it did not affect the main performance characteristics.

Intake, digestibility, growth performance, and enteric methane emission of Brazilian semiarid non-descript breed goats fed diets with different forage to concentrate ratios.

The aim of this study was to evaluate the intake, digestibility, growth performance, and enteric methane emissions of Brazilian semiarid non-descript breed goats (NDG) fed diets with different forage:concentrate ratios (100:0, 80:20, 60:40, 40:60, and 20:80) on a dry matter basis. Forty uncastrated male NDG with an average initial body weight of 13.3 kg ± 4.7 kg were distributed in a completely randomized design, with five treatments and eight replications. Ground Tifton-85 hay was used as forage and ground corn and soybean meal were used as concentrate. The sulfur hexafluoride tracer technique was used to measure methane emissions. The intake of dry matter, organic matter, crude protein, and ether extract increased linearly while the intake of neutral detergent fiber decreased linearly as the concentrate proportion increased (P < 0.05). The digestibility of dry matter and organic matter increased while the digestibility of neutral detergent fiber decreased as the concentrate level increased (P < 0.05). There were linear increases in final body weight, total weight gain, average daily gain, and feed efficiency (P < 0.0001). Methane emissions per unit of body weight (ranging from 1.9 to 0.5 g/kg), metabolic body weight (ranging from 3.9 to 1.2 g/kg), and dry matter intake (ranging from 58.8 to 21.9 g/kg) reduced linearly as the concentrate proportion increased (P < 0.01). Decreasing the forage to concentrate ratio in the diet decreased methane emission and increased growth performance of NDG. The 80:20 ratio could be considered more appropriate to reduce methane emissions from NDG, which did not change much at higher levels of concentrate.

Net requirements of calcium, phosphorus, magnesium, and sulphur for growth of non-descript breed hair lambs of different sex classes in the Brazilian semiarid conditions.

The aim of this study was to determine the net requirements of calcium (Ca), phosphorus (P), magnesium (Mg), and sulphur (S) for weight gain of non-descript breed hair lambs (NDBL) of different sex classes reared under Brazilian semiarid conditions. Sixty NDBL (20 intact males, 20 castrated males, and 20 females), with an average initial body weight of 18.1 ± 0.4 kg and average age of 5 months were used. The macromineral requirements were determined by the differences in body composition through comparative slaughter direct method. The animals were confined for 58 days, and the average fasting body weight (FBW) and average daily gain (ADG) of lambs fed ad libitum were 29.2 ± 0.6 and 0.19 ± 0.01 kg, respectively. The net requirements of macrominerals for gain of NDBL did not differ between sex classes (P > 0.05), and ranged from 1.83 to 1.46 g/day of Ca, 1.21 to 1.01 g/day of P, 37 to 30 mg/day of Mg, and 0.31 to 0.28 g/day of S, for lambs with an ADG of 0.200 kg and 15 or 30 kg of FBW, respectively (P < 0.05). The N to S net ratio reduced and increased, respectively, as the body weight and ADG increase. The net requirements of Ca, P, Mg, and S for gain of NDBL slaughtered young and at the same age did not differ between sex classes and decreased as the body weight increased. The net requirements for gain of Ca and P were similar to those recommended by NRC (2007).

Detoxified castor meal in substitution of soybean meal in sheep diet: growth performance, carcass characteristics and meat yield.

The aim of this study was to evaluate the intake, digestibility, performance, and carcass characteristics of lambs fed different levels of replacement (0, 15, 30, and 45 % based on dry matter, DM) of soybean meal (SM) by detoxified castor meal (DCM). Twenty-four and 32 intact hair lambs of nondescript breed (21.7 ± 2.6 kg of initial average body weight and approximately 10 months old) were used, respectively, in the intake and digestibility and performance experiments. The diets were composed of buffel grass hay, ground corn grain, and different levels of SM, DCM, and urea, in a roughage-to-concentrate ratio of 40:60. There was no effect of treatments on DM intake. However, crude protein (CP) and neutral detergent fiber (NDF) intakes were higher at 30 and 45 % than at 0 and 15 % of DCM, which in turn showed higher intake of non-fiber carbohydrates (NFC) (P < 0.05). The organic matter, CP, and NDF digestibilities were not affected, but the digestibility of NFC was lower at 30 and 45 % than at 0 % of DCM (P < 0.05). The average daily gain, feed conversion, slaughter and carcass weights, chilling losses, ribeye area, and absolute values and yields of neck, ribs, loin, and leg were not affected. However, the carcass yield was lower at 45 % of DCM and the absolute value of shoulder was lower at 30 and 45 % of DCM (P < 0.05). The replacement of SM by DCM up to 45 % in the feed of lambs did not negatively affect the intake, digestibility, performance, and main carcass features.

Mango tree pruning hay in substitution of elephant grass in cattle diet.

This experiment was conducted to evaluate the effect of different levels of substitution (0, 33.3, 66.7, and 100 %) of elephant grass by hay of mango tree pruning (HMTP) on intake and digestibility in cattle and on the in vitro gas production. Moreover, the effect of tannin in HMTP on the gas production and in vitro degradability of the dry matter was evaluated, using polyethylene glycol (PEG). The intake and digestibility were evaluated in a double 4 × 4 square Latin design, using eight Holstein intact bulls. To evaluate the gas production and degradability, in vitro semi-automated gas production technique was used. The substitution of elephant grass by HMTP decreased the intake (P<0.05) and the digestibility (P<0.05) of the nutrients and also the gas production (P<0.05). Furthermore, the utilization of PEG as a tannin-complexing agent increased the gas production and degradability of the HMTP (P<0.05). It was concluded that the high cell wall lignification and the presence of tannin limit the use of HMTP as the only source of roughage in cattle diets.